The Effect of Medium Supplementation with Second Carbon Source and Amino Acids for Enhanced Production of Cyclosporin A

Enhanced Production of Cyclosporin A Abstract The effect of a second carbon source and amino acids on the production of cylcosporin A (CyA) in a medium optimized by one factor at a time and orthogonal array method was studied. The one factor at-a-time method was adopted to investigate the effects of medium components (i.e. carbon, and nitrogen) and environmental factors (i.e. initial pH) on biomass growth and CyA production. Subsequently, L 16 orthogonal matrix was used to evaluate the significance of glucose, ammonium sulphate, K 2 HPO 4 and inoculum size. The effects of media components were ranked according to their effects on the production of CyA as glucose > ammonium sulphate > K 2 HPO 4 > inoculum size. The production increased from 58.35 mg/l to 148 mg/l when maltose (1 %) was added as a second carbon source after 8 days of fermentation. Evaluation of various amino showed that the addition of L-valine (6 g/l) and L-leucine (4 g/l) after 24 h fermentation enhanced the CyA production dramatically to 794 mg/l.